Bio-Safety Labs: Level 1 and Level 2

Biosafety Levels are a means to determine the level of danger (Bio-hazardous) to the researchers & others while working with biological / clinical samples that can cause diseases. Hence a clean environment is created in these laboratories through Air handling systems fitted with HEPA filters.

This facility could be used to probe materials and biological processes at the molecular and nanoscale to obtain information on the 3D structures and dynamics of molecules. This instrument is used by chemists, materials scientists, biologists and clinicians. NMR spectrometry is used to study everything from DNA to disease–causing proteins. Information obtained from NMR studies aids in the development of new drugs. NMR Spectroscopy is used to study molecular conformation in solution, quantitative analysis of mixtures, determining the content and purity of a sample.

BSL-2 facility, would be used to process clinical samples and biological specimens of Disease biology projects like Avascular Necrosis (AVN) of the femoral head, Huntington disease, Rheumatoid arthritis and Glaucoma.

• Interior volume 150L / 5.3 ft³
• Chamber construction Type 304 stainless steel, electropolished
• Temp. above ambient -55°C
• CO₂ Control: 0-20%

The BB 150 CO₂ incubator airflow patterns are designed for optimal distribution of critical environmental conditions. Efficient circulation minimizes variation between cultures while preventing desiccation.

Featuring reliable direct heat technology with high efficiency heaters on all sides of the culture chamber. In case of failure a backup circuit maintains control to ensure the stability of temperature at the desired value.

This provides automatic adjustment of the CO₂ measuring system at temperature set point with humidity, providing system optimization for best results. Advanced design of this CO₂ incubator promotes optimal growth & maintenance of cell cultures.

• Format: 384 well block
• Reaction Volume: 5-10 µl
• Multiplexing – Up to 5 targets
• Max. block ramp rate – 6.5°C/sec
• Average sample ramp rate – 3.66°C/sec
• Temp. Uniformity – 0.4°C
• Temp. accuracy – 0.25°C
• 384 wells peltier based heating and cooling
• OptiFlex® technology – 5 coupled excitation and emission filters with bright white LED as light source
• Advanced CMOS imager system for data acquisition
• Excitation/detection range – 450–650 nm/500–700 nm
• Heated cover temperature range of 50-110°C

• Run time: <30 min
• Dye Compatibility: FAM™/SYBR® Green, VIC®/JOE™/HEX/TET, ABY®/NED™/TAMRA™/ Cy®3, JUN®, ROX™/ Texas Red®, Mustang purple, Cy5/Liz, Cy5.5
• Can detect as low as 1 copy of and sense as small as 1.5-fold in target quantities

• Gene Expression, Genotyping, Copy number variation, Pathogen detection, Strain typing, and miRNA Profiling
• Protein thermal shift analysis

This facility is used to qualitatively measure the amplification of DNA/RNA using fluorescent dyes.

• Rotation range: 15 to 300 rpm
• Temp. Range: -15°C to 60°C

Offers sample storage and orbital shaking.

• Materials contaminated with pathogens requiring a defined biosafety level can be handled safely, ensuring personnel, sample and environment protection.
• This helps in preventing the escape of biological aerosols into the laboratory environment.

• 580mL capacity (4 x 145 mL)
• 99-program memory
• 15 optional rotor systems
• Powerful refrigeration system for precise control of temperature

• Aerosol biocontainment facility
• Runs up to 24 x 5/7mL blood tubes at a time in swinging bucket configuration
• Quick switch between applications- from conical tubes to blood tubes, and from microtubes to microplates

• Heating: 37 ± 0.5°C Stirrer: 150-850 RPM
• Pathlength: 10, 5, 2, 1 mm
• Detection Limit: 0.4 ng/µL dsDNA
• Maximum Concentration: 750 ng/µL

To quantify and assess purity of DNA, RNA and Protein.

• UV-VIS Mode:
  Excitation/Absorbance: 200-1000nm
  Light source: Xenon flash lamp
• Fluorescence Mode:
  Excitation/Absorbance: 200-1000nm
  Emission: 270-840nm
  Light source: Xenon flash lamp
• Time Resolved Fluorescence Mode:
  Excitation/Absorbance: Fixed at 334nm
  Emission: 400-700nm
  Light source: Xenon flash lamp
• Luminescence Mode:
  Excitation/Absorbance: 360-670nm

• Temp. range: From ambient +4°C to 45°C
• Shaking type: Orbital

• CO₂ concentration range: 0.1% to 15%
• CO₂ concentration stability: ±0.3% to 5% CO₂
• O₂ concentration range: 1% to 21%
• O₂ concentration stability: ±0.3% to 1% O₂

Reporter gene assays, Growth Kinetics, ATP measurement using Luciferin-Luciferase, Spectral scanning for assay optimization, Integrated gas module for atmospheric control of CO2 and O2 for cell based assays, Live cell calcium imaging studies and high throughput drug screening.